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Image Search Results
Journal: Cell Adhesion & Migration
Article Title: Lymphangiogenic responses of lymphatic endothelial cells to steady direct-current electric fields
doi: 10.1080/19336918.2023.2271260
Figure Lengend Snippet: Time course of phosphor-VEGFR-3 activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.
Article Snippet: In addition, phosphorylated VEGFR-3 in the cell lysates was measured using the RayBio
Techniques: Activation Assay, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Immunolabeling