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Time course of <t>phosphor-VEGFR-3</t> activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.
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Time course of <t>phosphor-VEGFR-3</t> activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.
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Time course of <t>phosphor-VEGFR-3</t> activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.
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R&D Systems human phospho erbb3 her3 elisa kit
Time course of <t>phosphor-VEGFR-3</t> activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.
Human Phospho Erbb3 Her3 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc phospho-her3 detection kit
Time course of <t>phosphor-VEGFR-3</t> activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.
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Image Search Results


Time course of phosphor-VEGFR-3 activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.

Journal: Cell Adhesion & Migration

Article Title: Lymphangiogenic responses of lymphatic endothelial cells to steady direct-current electric fields

doi: 10.1080/19336918.2023.2271260

Figure Lengend Snippet: Time course of phosphor-VEGFR-3 activation of HLECs after application of EF (200 mV/mm). Antiphospho-VEGFR-3 was detected using immunofluorescence staining and ELISA (see methods). EF-treated HLECs demonstrated immediate VEGFR-3 phosphorylation within 10 min. Protein expression was quantified using CLSM and ELISA. The images show representative immunolabeled cellular structures (a). The histograms depict the relative immunofluorescence (b) and OD from cell lysate (c) of the phosphorylated protein. Data were from three separate experiments. The error bars represent the standard error (SE). * P < .05, ** P < .01, *** P < .001, significantly different from the untreated control (0 minutes). Initial magnification of the images: ×200.

Article Snippet: In addition, phosphorylated VEGFR-3 in the cell lysates was measured using the RayBio Human Phosphotyrosine VEGFR-3 ELISA Kit (Cat# PEL-VEGFR-3-Y; RayBiotech, Inc., USA), according to the manufacturer’s protocol.

Techniques: Activation Assay, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Immunolabeling